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cd25 bv421 (clone bc96) antibody  (Sony)

 
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    Sony cd25 bv421 (clone bc96) antibody
    Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and <t>CD25</t> + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.
    Cd25 Bv421 (Clone Bc96) Antibody, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25 bv421 (clone bc96) antibody/product/Sony
    Average 90 stars, based on 1 article reviews
    cd25 bv421 (clone bc96) antibody - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation"

    Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.1039166

    Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and CD25 + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.
    Figure Legend Snippet: Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and CD25 + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.

    Techniques Used: Sampling, Standard Deviation

    Basal calcium level is elevated in B cells from ATA+, euthyroid, infertile patients Baseline median fluorescence intensity of Fluo4 in B cell subpopulations (A) , CD25- (C) and CD25+ (E) B cells. Cross tabulation shows the p values when comparing patient groups without the subpopulation-based grouping of B cells (B) , CD25- (D) and CD25+ cells (F) . HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons, where a mixed-effects analysis and the Sidak’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are presented as follows: middle line represents the median, dot represents the mean, box: interquartile range, whiskers: min-max. *p < 0.05, **p < 0.01; Significant p values are red, non-significant p values are written in blue.
    Figure Legend Snippet: Basal calcium level is elevated in B cells from ATA+, euthyroid, infertile patients Baseline median fluorescence intensity of Fluo4 in B cell subpopulations (A) , CD25- (C) and CD25+ (E) B cells. Cross tabulation shows the p values when comparing patient groups without the subpopulation-based grouping of B cells (B) , CD25- (D) and CD25+ cells (F) . HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons, where a mixed-effects analysis and the Sidak’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are presented as follows: middle line represents the median, dot represents the mean, box: interquartile range, whiskers: min-max. *p < 0.05, **p < 0.01; Significant p values are red, non-significant p values are written in blue.

    Techniques Used: Fluorescence, Sampling

    Naive B cells show higher Ca 2+ signal upon BCR stimulation in ATA+ euthyroid, infertile patients Calcium flux kinetic data of naive B, naive CD25- and naive CD25+ B cells (A) maximum value, (B) ending value, (C) time to reach maximum, (D) time to first 50% value, (E) time from first 50% to maximum, (F) time from maximum to second 50% value, (G) slope at the first 50% value, (H) slope at second 50% value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Figure Legend Snippet: Naive B cells show higher Ca 2+ signal upon BCR stimulation in ATA+ euthyroid, infertile patients Calcium flux kinetic data of naive B, naive CD25- and naive CD25+ B cells (A) maximum value, (B) ending value, (C) time to reach maximum, (D) time to first 50% value, (E) time from first 50% to maximum, (F) time from maximum to second 50% value, (G) slope at the first 50% value, (H) slope at second 50% value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Techniques Used: Software, Sampling, Standard Deviation

    Memory B cells of ATA+, euthyroid, infertile patients have increased calcium flux upon BCR activation Selected calcium flux kinetic data of memory B cell subsets: Non-switched (NSw) memory B cells, CD25- and CD25+ NSw cells: (A) maximum value, (B) ending value, (C) area under curve (AUC) value. Immunoglobulin class-switched (Sw) memory B cells, CD25- and CD25+ Sw cells: (D) maximum value, (E) ending value, (F) area under curve (AUC) value. CD27-, IgD- double-negative (DN) memory B cells, CD25- and CD25+ DN cells: (G) maximum value, (H) ending value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14). The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Figure Legend Snippet: Memory B cells of ATA+, euthyroid, infertile patients have increased calcium flux upon BCR activation Selected calcium flux kinetic data of memory B cell subsets: Non-switched (NSw) memory B cells, CD25- and CD25+ NSw cells: (A) maximum value, (B) ending value, (C) area under curve (AUC) value. Immunoglobulin class-switched (Sw) memory B cells, CD25- and CD25+ Sw cells: (D) maximum value, (E) ending value, (F) area under curve (AUC) value. CD27-, IgD- double-negative (DN) memory B cells, CD25- and CD25+ DN cells: (G) maximum value, (H) ending value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14). The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Techniques Used: Activation Assay, Software, Sampling, Standard Deviation



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    cd25 bv421 (clone bc96) antibody  (Sony)
    90
    Sony cd25 bv421 (clone bc96) antibody
    Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and <t>CD25</t> + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.
    Cd25 Bv421 (Clone Bc96) Antibody, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd25 bv421 (clone bc96) antibody/product/Sony
    Average 90 stars, based on 1 article reviews
    cd25 bv421 (clone bc96) antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and CD25 + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

    doi: 10.3389/fimmu.2022.1039166

    Figure Lengend Snippet: Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and CD25 + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.

    Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

    Techniques: Sampling, Standard Deviation

    Basal calcium level is elevated in B cells from ATA+, euthyroid, infertile patients Baseline median fluorescence intensity of Fluo4 in B cell subpopulations (A) , CD25- (C) and CD25+ (E) B cells. Cross tabulation shows the p values when comparing patient groups without the subpopulation-based grouping of B cells (B) , CD25- (D) and CD25+ cells (F) . HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons, where a mixed-effects analysis and the Sidak’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are presented as follows: middle line represents the median, dot represents the mean, box: interquartile range, whiskers: min-max. *p < 0.05, **p < 0.01; Significant p values are red, non-significant p values are written in blue.

    Journal: Frontiers in Immunology

    Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

    doi: 10.3389/fimmu.2022.1039166

    Figure Lengend Snippet: Basal calcium level is elevated in B cells from ATA+, euthyroid, infertile patients Baseline median fluorescence intensity of Fluo4 in B cell subpopulations (A) , CD25- (C) and CD25+ (E) B cells. Cross tabulation shows the p values when comparing patient groups without the subpopulation-based grouping of B cells (B) , CD25- (D) and CD25+ cells (F) . HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons, where a mixed-effects analysis and the Sidak’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are presented as follows: middle line represents the median, dot represents the mean, box: interquartile range, whiskers: min-max. *p < 0.05, **p < 0.01; Significant p values are red, non-significant p values are written in blue.

    Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

    Techniques: Fluorescence, Sampling

    Naive B cells show higher Ca 2+ signal upon BCR stimulation in ATA+ euthyroid, infertile patients Calcium flux kinetic data of naive B, naive CD25- and naive CD25+ B cells (A) maximum value, (B) ending value, (C) time to reach maximum, (D) time to first 50% value, (E) time from first 50% to maximum, (F) time from maximum to second 50% value, (G) slope at the first 50% value, (H) slope at second 50% value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

    doi: 10.3389/fimmu.2022.1039166

    Figure Lengend Snippet: Naive B cells show higher Ca 2+ signal upon BCR stimulation in ATA+ euthyroid, infertile patients Calcium flux kinetic data of naive B, naive CD25- and naive CD25+ B cells (A) maximum value, (B) ending value, (C) time to reach maximum, (D) time to first 50% value, (E) time from first 50% to maximum, (F) time from maximum to second 50% value, (G) slope at the first 50% value, (H) slope at second 50% value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

    Techniques: Software, Sampling, Standard Deviation

    Memory B cells of ATA+, euthyroid, infertile patients have increased calcium flux upon BCR activation Selected calcium flux kinetic data of memory B cell subsets: Non-switched (NSw) memory B cells, CD25- and CD25+ NSw cells: (A) maximum value, (B) ending value, (C) area under curve (AUC) value. Immunoglobulin class-switched (Sw) memory B cells, CD25- and CD25+ Sw cells: (D) maximum value, (E) ending value, (F) area under curve (AUC) value. CD27-, IgD- double-negative (DN) memory B cells, CD25- and CD25+ DN cells: (G) maximum value, (H) ending value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14). The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

    doi: 10.3389/fimmu.2022.1039166

    Figure Lengend Snippet: Memory B cells of ATA+, euthyroid, infertile patients have increased calcium flux upon BCR activation Selected calcium flux kinetic data of memory B cell subsets: Non-switched (NSw) memory B cells, CD25- and CD25+ NSw cells: (A) maximum value, (B) ending value, (C) area under curve (AUC) value. Immunoglobulin class-switched (Sw) memory B cells, CD25- and CD25+ Sw cells: (D) maximum value, (E) ending value, (F) area under curve (AUC) value. CD27-, IgD- double-negative (DN) memory B cells, CD25- and CD25+ DN cells: (G) maximum value, (H) ending value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14). The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

    Techniques: Activation Assay, Software, Sampling, Standard Deviation